There are numerous points during library preparation that can result in biased results. Our products allow you to better understand your pipeline's limitations and identify areas for improvement. Our library prep bias products can help you to assess:

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1. GC Bias: sequences with high GC content can be particularly difficult to 

2. Length Bias: There can be numerous areas during library preparation that can result in biases against larger or shorter sequences. 

3. Cross contamination: ​​A better understanding of laboratory derived contamination (during sample processing) or contamination during sequencing (index hopping) can provide invaluable insights into possible false positive rates and help identify areas for assay improvement. 

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Explore our products to understand how they can help you assess library preparation biases.

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Products to consider:

• Kelomix 1

Accuracy is the measure of how closely the output of a method matches the true input. The definition of accuracy differs depending on the test that is being performed:

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1. Qualitative Accuracy:

2. Quantitative Accuracy: 

Analytical sensitivity refers to the lowest amount of an analyte that can be detected. Typically sensitivity refers to the number of positives detect in a sample at a specific amount divided by the number of true positives. For NGS assays this can be done in two ways:

1. Relative sensitivity: also referred to as qualitative or semi-quantitative sensitivity is the lowest amount of an analyte that will result in a correct call. Keloma offers several mixes to help you determine the relative sensitivity in terms of parts per million. Relative sensitivity is particularly useful when sequencing depth and other factors vary considerably. 

2. Quantitative sensitivity: is the lowest amount of an analyte that you are able to detect. For a quantitative test this is a defined amount. Keloma offers several products capable of quantitatively assessing a methods sensitivity in terms of attomoles/ul.

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We offer several products that can assess both relative and quantitative sensitivity

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Products to consider:

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Specificity refers to the ability of a method to exclusively measure the target analyte, this is typically a measure of the number of negatives detected divided by true negatives. Keloma offers a wide range of products with known analytes that can be used to assess method specificity.